Towards antibacterial endodontic sealers using quaternary ammonium nanoparticles.

AIM To change and characterize the antibacterial properties of endodontic sealers by incorporating low concentrations of insoluble antibacterial nanoparticles (IABN). METHODOLOGY The antibacterial effect against Enterococcus faecalis was evaluated by (i) agar diffusion test (ADT), (ii) direct contact test (DCT) and (iii) scanning electron microscopy (SEM). IABN were incorporated into AH Plus (Dentsply, DeTrey Konstanz, Germany) and GuttaFlow (Coltène Whaledent, Langenau, Germany) at concentrations of 0.5%, 1% or 2% weight/weight. Bacterial growth rates were analysed using ANOVA followed by Tukey's test. RESULTS The antibacterial tests demonstrated total bacterial growth inhibition using AH Plus samples incorporating 2% weight/weight IABN after 4 weeks (P < 0.005). DCT showed total growth inhibition of up to 6 logs in viable count in AH Plus samples incorporating IABN and up to 4 log in count in GuttaFlow incorporating IABN (P < 0.005). Significant differences were found between the unmodified sealers and the experimental groups. No antibacterial effect was observed in the ADT, indicating IABN were not diffusing into the agar. Furthermore, SEM indicated bacterial cell wall damage and lysis. CONCLUSIONS AH Plus and GuttaFlow incorporating low concentrations of IABN exhibited significant and stable antimicrobial properties.